46,XY/45,X mosaicism in an amniotic fluid cell culture: suppression of abnormal cell line after subcultivation.

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46,XY/45,X mosaicism in an amniotic fluid cell culture: suppression of abnormal cell line after subcultivation.

An abnormal cell population, 45,X, appeared in 3 of 4 cell lines established from an amniotic fluid specimen obtained from a normal mid-trimester pregnancy. Two of the cell lines were subjected to repeated chromosome analyses until VII passage. The abnormal cells were suppressed after repeated trypsinisations; simultaneously, fibroblast-like cells outgrew the cultures, which were previously pre...

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Observations on human amniotic fluid cell strains in serial culture.

Observations made on 31 amniotic fluid cell strains serially cultured until senescent are recorded. The cell strains had an average life in culture of 13-9 passages (range 3-29). The source of the amniotic fluid from which the cultures were initiated did not influence the behaviour of the cell strains. The behaviour ofthe cell strains was unrelated to the growth characteristics of the primary c...

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amniotic cell culture

A metacentric bisatellited microchromosome was detected in all metaphases from an amniotic culture performed because of maternal age. A wide-ranging survey of the literature failed to disclose any consistent anomaly associated with such a marker, but did reveal that the clinical picture of patients manifesting it could range from complete normality through mental retardation to a variety of def...

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Amniotic fluid cell cultures.

In an 18-month period, 249 samples of amniotic fluid were used experimentally in attempts to enhance success in cell cultures. None ofthe experimental situations proved entirely satisfactory in this aim. Emphasis is laid on the quality ofserum used in culture. Particularly satisfactory serum should be retained for use in amniotic fluid cell cultures which are dependent on unknown growth stimula...

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RESULTS OF CYTOGENETIC ANALYSIS OF 521 AMNIOTIC FLUID CELL CULTURES (AMNIOCENTESES) PERFORMED IN IRAN

The indications and results of cytogenetic analysis of 521 amniotic cell cultures performed at 13-16 weeks of gestation were evaluated in this study. 507 fetuses (97.3%) were cytogenetically normal, 14 (2.7%) had unbalanced karyotypes, and 2 fetuses were found to have major abnormalities, one with anencephaly detected by measurement of alpha -fetoprotein levels in amniotic fluid and ultraso...

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ژورنال

عنوان ژورنال: Journal of Medical Genetics

سال: 1979

ISSN: 1468-6244

DOI: 10.1136/jmg.16.4.267